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SHG imaging of optical clearing in tendon. Forward SHG attenuation data for the uncleared and cleared tendon (5 h treatment with 50% glycerol). Clearing results in increased swelling and a decrease in the rate of the axial attenuation. Integration under the curves reflects the entire SHG intensity before and after clearing, in which the SHG enhancement factor was ~2×. Reproduced from ref. 45 with permission from SPIE Publications.
