Figure 3. Lung CD103⁺CD8⁺ T cells are CD45R0⁺ effector or memory cells.

Differentiation stages of the CD8⁺ T cells within the CD103⁺ and CD103^– cell fractions as assessed by flow cytometry. Naive cells were defined as CD27⁺CD45R0^–, memory cells were defined as CD27⁺CD45R0⁺, and effector cells were defined as CD27^–CD45R0^±. (A) Representative FACS plots for CD103⁺ and CD103^–CD8⁺ T cells. Dot plots are gated on CD3⁺CD8⁺CD103⁺ cells among live lymphocytes or on the CD3⁺CD8⁺CD103^– cells among live lymphocytes. Numbers indicate the percentages of CD3⁺CD8⁺CD103⁺ or CD3⁺CD8⁺CD103^– cells that are located within each quadrant. (B) Naive CD8⁺ T cells (CD27⁺CD45R0^–), memory CD8⁺ T cells (CD27⁺CD45R0⁺), effector/memory (EM) CD8⁺ T cells (CD27^–CD45R0^±), and CD45R0^–CD8⁺ T cells as percentages of total CD103⁺ and CD103^–CD8⁺ T cell fractions (n = 28). ***P < 0.0001.