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. 2011 May 23;121(6):2181–2196. doi: 10.1172/JCI44771

Figure 6. Hyperactivation of mTORC1 leads to mislocalization of slit diaphragm proteins.

Figure 6

(A) Nephrin expression at interpodocyte filtration slit area is diminished in PcKOTsc1 mice. Representative electron micrographs of immunogold nephrin staining in 3-week-old mice are shown. Number of immunogold particles for nephrin at the slit diaphragm was measured in 290 slit diaphragms from 2 wild-type and 170 slit diaphragms from 2 PcKOTsc1 mice. *P < 0.001 versus wild-type, mean ± SEM. (B) Nephrin and actin expression in the indicated glomeruli were determined by Western blotting. Levels of nephrin expression in the indicated genotypes were quantified (nephrin/actin). Data are expressed as fold increase. P = 0.67, mean ± SEM, n = 3. (C) Nephrin is mislocalized in PcKOTsc1 podocytes. Confocal microscopic analyses of nephrin and synaptopodin expression in the indicated animals (3 weeks old) are shown. Rapamycin treatment was performed for 1 week. Areas indicated by squares in the Merge column are shown at higher magnification in the right two columns. Original magnification, ×13,500 (A), ×400 (C).