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. 2011 May 9;121(6):2350–2360. doi: 10.1172/JCI46102

Figure 3. Gene expression of circulating CD8+ T cells depending on antigen specificity.

Figure 3

(A and B) Volcano plots for all gene probes, showing differential expression and P values of the comparison of tumor- versus EBV-specific T cells (A) or tumor- versus CMV-specific T cells (B); diagramming is similar to that in Figure 1. (C and D) Two-way hierarchical clustering based on the identified gene probes separating all tumor-specific T cells from EBV- (C, 405 gene probes corresponding to 390 genes) and from CMV-specific T cells (D, 187 gene probes corresponding to 184 genes). Red indicates overexpression and blue underexpression relative to the mean. Each row represents 1 gene and each column 1 1,000-cell sample from 1 patient (tumor-, EBV- and CMV-specific cells) or healthy donor (EBV-specific cells, n = 4). (E and G) Log fold changes between tumor- and EBV- (E) or tumor- and CMV-specific T cells (G) of data from microarrays (blue bars) and qPCR (red bars). Positive and negative values indicate overexpression in tumor- and in virus-specific T cells, respectively. Data are represented as mean ± SEM. (F and H) Relative overexpression of GO terms associated with the identified 390 genes (Melan-A/MART-1 versus EBV; F) or with the identified 184 genes (Melan-A/MART-1 versus CMV; H), calculated as described in Methods.