Figure 5. Angpt1 regulates tissue response in wound healing.

(A) Wound closure in ear punch wounds is increased in Angpt1^{del/^(E13.5)} mice. (B) Cross sections of one of the sides of the ear punch show the cut of cartilage and the larger closed zone in Angpt1^{del/^(E13.5)} mice (Masson Trichrome) (original magnification, ×100). (C) Quantification (mean ± SEM) of the punch area after 60 days shows a significant decrease in open area in the Angpt1^{del/^(E13.5)} mice. (D) Z-stacks of flat mounted ears stained for CD31 (red, endothelium) and Ng2 (green, pericytes) show increased angiogenesis in the closed zone of Angpt1^{del/^(E13.5)} mice compared with that of controls (scale bar: 100 μm). Z-stacks are composed of 8 images every 3 μm. The thick line (only in control) is the start of the closed zone (cut), and the thin line is the edge of the closed zone. (E) The close up of vessels in the healed area shows normal pericyte coverage (Desmin, green) of vessels (scale bar: 10 μm).