Figure 2.

Spatial-density distribution of the FG-repeat regions in the NPC. (A) Map density of Nups derived from the combined localization volumes of all structured domains (five different colors) and the normalized localization probability of all unstructured regions (green cloud). The estimated unstructured regions are based on integrated data from cryo-EM, immuno-electron microscopy, ultracentrifugation, immunoblotting, protein interaction assays, affinity purification and proteomics.⁵⁰,⁵¹ NE indicates the location of the nuclear envelope. (B) Projection of the localization probabilities of the FG-repeat regions from all the FG-Nups is shown by a density plot. Red for the FG-Nups that are cytoplasmically disposed; blue for those nucleoplasmically disposed; white for those found equally on both sides. (A and B) are reprinted from reference 51 with permission from the publisher. (C) Projection of the normalized spatial-density map of interactions between Imp β1 and the FG repeats (green cloud) at the central pore region, detected by SPEED microscopy. (D) The same density map as in (C) is shown in different colored regions (red for the cytoplasm, blue for the nucleoplasm and green for the central pore).