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Immunostaining of the epichromatin epitope through mitosis in U2OS (A) and HL-60/S4 (B) cells. Mouse mAb PL2-6 staining is shown in red; DAPI staining in blue. Each image is a single deconvolved optical slice. Bar equals 10 µm for both (A and B). Projection videos for some of these images of U2OS can be found in the Supplementary Video Files, specifically: late prophase (Video 1); early anaphase (Video 2); late anaphase (Video 3); early telophase (Video 4); late telophase (Video 5).
