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. 2011 Jan-Feb;2(1):47–60. doi: 10.4161/nucl.2.1.13271

Figure 3.

Figure 3

Co-immunostaining of U2OS interphase and metaphase cells with the epichromatin, mitotic marker and centromere (CREST) antibodies. The mitotic cell is on top of each part; the interphase cell is at the bottom. (A) mouse mAb PL2-6; (B) rabbit anti-H3 phosphorylated at serine 10, the mitotic marker H3(S10)p; (C) DAPI; (D) merge with an insert showing a 3-fold enlargement of the region denoted by an asterisk; (E) merged image of PL2-6, CREST and DAPI. Mouse mAb PL2-6 staining is shown in red, H3(S10)p and CREST in green, DAPI in blue. Each part is a single deconvolved optical slice of the same field. Bar equals 10 µm.