Figure 9.
Immunostaining of the epichromatin epitope in tobacco and Arabidopsis thaliana cells. (A–D), confocal sections of mitotic stages seen in tobacco BY-2 cells immunostained with mAb PL2-6 (red): (A) interphase; (B) metaphase plate; (C) anaphase; (D) telophase. (E) confocal section of a whole mount of a Arabidopsis root tip stained with PL2-6 (red). (F) electron micrograph of a post-embedded immunogold stained thin section of a high pressure freezing/freeze substituted Arabidopsis root tip. The arrows point to the 5 nm gold near the NE. The astericks indicate the position of nuclear pores. CW, cell wall; C, cytoplasm; N, nucleus. Magnifications: (A–D), bar in (D) equals 10 µm; (E) bar equals 10 µm; (F) bar equals 200 nm.