Fig. 4.

Eosinophilia in peripheral blood of rac2−/− C57Bl/6 mice. Discombe's staining of peripheral blood eosinophils was performed on 4–6 mice/group in each experiment; a representative result from 3 experiments is shown. The total number of mice used in 3 independent experiments with different blood sample sources was 18 wild-type and 14 rac2−/− mice. These experiments could not be combined into a single graph, since blood was collected from different parts of the body (femoral, retroorbital), which leads to different total eosinophil numbers in wild-type mice [51]. Wild type refers to C57Bl/6 mice (not IL-5 Tg), rac2−/− refers to rac2−/− mice on the C57Bl/6 background. ∗ p < 0.05.