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Determination of the mutation of T103N and N184Q at T11 peptide from the trypsin plus PNGase F digest of TNK-tPA by the LC-MS analysis. The LC-MS elution profile (A), the precursor mass of the deglycosylated T11 by FTICR (B), and the CID-MS2 of the precursor (C), with the annotation of the identified peptide sequence indicated in the inserts.
