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. Author manuscript; available in PMC: 2011 May 31.
Published in final edited form as: Anal Chem. 2010 Jul 15;82(14):6154–6162. doi: 10.1021/ac100956x

Figure 4.

Figure 4

Determination of the glycosylation occupancy at N184 of T17 peptide from the trypsin plus PNGase F digest of TNK-tPA. The extracted ion chromatography of D (aspartic acid) form representing the glycosylation occupancy and N (asparagine) form representing the non-glycosylation occupancy of T17 are shown as Figures 4A and 4B, respectively, with the accurate precursor masses representing for D and N forms indicated in the inserts. The % of glycosylation occupancy was estimated by the ratio of D divided by the sum of D and N peptide intensities, as indicated in the bottom of the figure.