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. Author manuscript; available in PMC: 2011 May 31.
Published in final edited form as: Anal Chem. 2010 Jul 15;82(14):6154–6162. doi: 10.1021/ac100956x

Figure 5.

Figure 5

Figure 5

Figure 5A. Comparison of the reduced and alkylated TNK-tPA from the innovator and follow-on manufacturers by SDS-PAGE separation. The resolved single and 2-chain forms with their associated glycosylation such as type I (3 glycosylation sites) and type II (2 glycosylation sites) are indicated with squares and arrows in the figure.

Figure 5B. Comparison of the reduced and alkylated TNK-tPA (with and without PNGase F treatment) from the innovator and follow-on manufacturers by SDS-PAGE separation. The resolved single and 2-chain forms with their associated glycosylation such as type I (3 glycosylation sites) and type II (2 glycosylation sites) or deglycosylated forms (with PNFase F treatment) are indicated with squares and arrows in the figure.