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Determination of the extent of 2-chain cleavage from the Lys-C digest of TNK-tPA. The extracted ion chromatography of the cleaved (2-chain) and un-cleaved (single-chain) peptides are shown as Figures 6A and 6B, respectively. The % of 2-chain was estimated by the ratio of 2-chain divided by the sum of 2-chain and single-chain peptide intensities, as indicated in the bottom of the figure.
