. Author manuscript; available in PMC: 2011 May 31.

Published in final edited form as: Anal Chem. 2010 Jul 15;82(14):6154–6162. doi: 10.1021/ac100956x

Table 1.

Determination of glycosylation occupancy of TNK-tPA at N184 site using either PNGase F treatment for tryptic glycopeptides or in-gel digestion of the resolved glycosylated and non-glycosylated intact proteins.

Product / Method	Innovator	Follow-on
PNGase F treatment (% occupancy at N184)	60 (±3) %	35 (±2) %
In-Gel Analysis (% occupancy at N184)	50 (±5) %	20 (±3) %

Non-glycosylated and deglycosylated tryptic peptides and sites:

T17 (163–189): YSSEFCSTPACSEGNSDCYFGDGSAYR (deglycosylated)

T17 (163–189): YSSEFCSTPACSEGNSDCYFGNGSAYR (non-glycosylated)

Average of 3 measurements