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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 25;79(5):1182–1193. doi: 10.1111/j.1365-2958.2011.07540.x

Fig. 1.

Fig. 1

Artificially controlled expression of NifB in A. vinelandii. A, comparison of physical maps of A. vinelandii chromosomal regions around the nifB locus in the wild-type strain (DJ), a ΔnifB strain (UW140), and an IPTG-controlled gst::nifB-expressing strain (UW233). B, Immunoblot developed with antibodies to NifB showing accumulation of NifB or GST-NifB in A. vinelandii strains, DJ, UW140 and two clones of strain UW233. All strains were derepressed for nitrogen fixation during 4 h; in addition, UW233 cultures were treated with 1 mM IPTG. C, GST-NifB accumulation in strain UW233 under nitrogenase derepressing conditions in medium supplemented with increasing concentrations of IPTG.