Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 25;79(5):1182–1193. doi: 10.1111/j.1365-2958.2011.07540.x

Fig. 3.

Fig. 3

Effect of ΔnifENX and ΔnifA mutations on the degradation rates of GST-NifB protein. Immunoblot analyses of GST-NifB accumulated under diazotrophic or ammonium-amended medium in A. vinelandii strains UW233 (A), UW238 (B), and UW295 (C). UW238 is a ΔnifENX::kn derivative of UW233. UW295 is a ΔnifA::sp derivative of UW233. A. vinelandii cells cultured in medium containing ammonium (Unind) were induced for GST-NifB expression during 2 h by adding IPTG to the medium (Ind). Cells were then treated as described in Fig. 2B and analyzed by immunoblot developed with antibodies to NifB. The asterisk points to a GST-NifB-degradation product. Densitometric quantitations of GST-NifB disappearance under ammonium-dependent (squares) or diazotrophic (circles) growing conditions are shown for each strain.