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. 2011 May 31;5(5):e1166. doi: 10.1371/journal.pntd.0001166

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Left panel, SDS-PAGE of soluble and insoluble proteins extracted from adult S. japonicum and recombinant proteins. Right panel: Recognition of recombinant Sj-TSP-2e and native proteins by antibodies in hyper immune mouse serum raised against recombinant Sj-TSP-2e. Lane M, protein markers; Lanes 1 and 2, soluble and insoluble proteins of S. japonicum; lane 3, Thi; lane 4, Sj-TSP-2e-Thi; lane 5, Sj-TSP-2e expressed in Pichia yeast; lane 6, Sm-TSP-2e-Thi; lane 7, Sj-23-GST-His as a control protein.