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. 2011 May 31;5(5):e1166. doi: 10.1371/journal.pntd.0001166

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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I: ELISA IgG screen of 72 sera collected from positive schistosomiasis japonica patients and 24 normal human sera (negative controls) probed by protein preparation from S. japonicum adult worms (SWAP), recombinant tag protein thioredoxin (Thio) and Sj-TSP-2e. II. Panel a: SDS-PAGE of soluble and insoluble protein extracted from adult S. japonicum and recombinant Sj-TSP-2e. Panels b and c: Western blot analysis with pooled sera randomly selected from confirmed schistosomiasis japonica patients (n = 15) and pooled sera from negative control subjects (n = 15) from northern China. Lane M, protein markers; lanes 1 and lane 2, soluble and insoluble proteins from adult S. japonicum; lane 3, Sj-TSP-2e (arrowed); lane 4, thioredoxin tag (Thi) (arrowed); lane 5, Sj-TSP-2e expressed in Pichia yeast (arrowed).