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. 2011 May 31;6(5):e20049. doi: 10.1371/journal.pone.0020049

Figure 5. Restriction map of the mtDNA ND5 amplicon.

Figure 5

The ApoI site at position 344 distinguishes species [11] of the tenebrosa group (European Daphnia pulicaria, EuroPC; and D. tenebrosa, TENE) from those of the pulicaria group (polar, PPC; western, WPC; and eastern D. pulicaria, EPC; D. middendorffiana, MIDD; and panarctic D. pulex, PanPX). Some lineages yield short fragments under 100 bp in size difficult to visualize using agarose gels but this does not affect scoring the individuals as either tenebrosa or pulicaria group.