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. 2011 May 31;6(5):e19570. doi: 10.1371/journal.pone.0019570

Figure 4. Anti-TgRSC8 antibodies detect a 84kDa nuclear protein.

Figure 4

A. Anti-TgRSC8 monoclonal antibodies 3AD6 and 1DE10 detect an 84 kDa protein in extracts of either tachyzoites (T) or bradyzoites (B) of the vector control stain VC2 by western immunoblot. Markers of molecular mass are shown at the right in kDa. Blots were reprobed to indicate reactivity of β-tubulin (TUB2) and BAG1 as controls for loading and bradyzoite transition, respectively. B. TgRSC8 is a nuclear protein in both tachyzoites and bradyzoites. TgRSC8 was detected in wild-type parasites using 1DE10 antibody (green). DNA is detected by DAPI (blue). Tachyzoites are indicated by detection of the surface marker SAG1, while bradyzoites are shown by Dolichos biflorus agglutinin (DbA) reactivity (red). At left are DIC images, including a 5 µm scale indicator (black bar), and merged panels are shown on the right.