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. 2011 May 31;6(5):e19570. doi: 10.1371/journal.pone.0019570

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Rooney et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Q-PCR was performed on cDNA derived from in vitro bradyzoites of vector control strains VC1 (white bars) and VC2 (gray bars), TgRSC8 mutant C9 (black bars), and three complemented strains (comp1-3, patterned bars) to analyze transcript levels of bradyzoite-induced genes BAG1, LDH2 (panel A), SUSA1, ENO1 (panel B), a bradyzoite-induced HSP70, BRP1, BGR1 and SAG2X (panel C). Transcripts of constitutive genes GRA2 and GAPDH were also quantified (panel D). Transcript levels were expressed as a percentage of the level of housekeeping gene TUB1 per sample. Shown are the averages and standard deviations of three experiments, performed on cDNAs derived from independent RNA samples.