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. 2011 May 31;6(5):e20208. doi: 10.1371/journal.pone.0020208

Figure 4. Single incorrect nt incorporation and K65R production rates with subtype B RT on subtype B and C templates.

Figure 4

(A) Lanes 1 through 10 depict (+)dsDNA synthesis from the (−)ssDNA intermediate with subtype B RT on the subtype B template. The full-length product is observed as a single dGTP incorporation occurred at the P+1nt position. Lanes 11 through 20 depict (+)dsDNA synthesis from the (−)ssDNA intermediate with subtype B RT on the subtype C template. The full-length product is observed as containing two distinct dGTP incorporations at the P+1nt and P+2nt positions, indicative of dislocation with the subtype C template. (B) Graphical representation of transcript production with subtype B RT on both the subtype B and C templates. The values indicated with an asterisk have a p-value <0.05 when the amount of transcripts containing the mutagenic nt produced between both subtypes at the given time-point is compared. More transcripts containing the mutagenic G nt at codon 65 are produced with the subtype C template than with the subtype B template. (C) Depiction of the primer and template systems used. dGTP was the only nt employed in the reaction to allow for the production of the mutagenic transcripts. The homopolymeric regions of both templates are underlined and the base responsible for the K65R mutation is indicated in bold.