Skip to main content
. 2011 May 31;6(5):e20385. doi: 10.1371/journal.pone.0020385

Figure 6. Cytokine expression in SEA-restimulated lymph node cultures from sham and CLP RAG mice.

Figure 6

Single-cell suspensions of lymph nodes from S. mansoni-sensitized sham and CLP RAG mice four days following SEA-bead challenge were restimulated with soluble SEA in cell culture for 48 hours, and cytokine expression in cell culture supernatants was analyzed via multiplex bead assay (Luminex). Data presented is representative of two separate experiments, with triplicate wells of samples from sham and CLP RAG mice, n = 5 mice per group. The limit of detection for each cytokine was routinely <5 pg/ml. (*) = p<0.05 vs. sham RAG, SEA-stimulated.