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. 2011 May 31;6(5):e20307. doi: 10.1371/journal.pone.0020307

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Kong, Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The solubility of fusion proteins was analyzed on 12% SDS-PAGE gel and stained with Coomassie Brilliant blue. M: protein molecular weight marker, lane 1: total cellular lysate from E. coli containing pET/SUMO, lane 2: soluble lysate fraction from E. coli containing pET/SUMO, lane 3: insoluble lysate fraction from E. coli containing pET/SUMO, lane 4: total cellular lysate from E. coli containing pET/SUMOtFgf15, lane 5: soluble lysate fraction from E. coli containing pET/SUMOtFgf15, lane 6: insoluble lysate fraction from E. coli containing pET/SUMOtFgf15.