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. 2011 May 31;6(5):e20307. doi: 10.1371/journal.pone.0020307

Figure 4. Purification of SUMOtFgf15 inclusion bodies (A), confirmation of SUMOtFgf15 protein refolding following ScUlp1 digestion (B).

Figure 4

Panel A lane 1: soluble cell lysate from pET/SUMOtFgf15, lane 2: insoluble inclusion bodies, lane 3: unbound protein after Ni-NTA resin, lane 4: elutes from Ni-NTA by 200 mM imidazole, lane 5: soluble protein after refolding. Panel B lane 1: purified SUMOtFgf15 for starting refolding, lane 2: soluble SUMOtFgf15 protein after refolding, lane 3: refolded SUMOtFgf15 digested by ScUlp1 for 30 mins. Panel C shows the expression and purification of protease ScUlp1. Lane 1–2: lysate from E. coli containing pET28a(+) (lane 1, soluble fraction, lane 2: insoluble fraction), lane3–4: lysate from E. coli containing pET/ScUlp1 (lane 3: soluble fraction, lane 4: insoluble fraction), lane 5: unbound protein after Ni-NTA resin, lane 6: eluted ScUlp1 by 100 mM imidazole, lane 7: eluted ScUlp1 by 200 mM imidazole.