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A fraction of SecA-FM (A) and proOmpA-Atto647N in complex with the chaperone SecB (B) bind non-specifically to the OG8 fibers. SecA-FM (C) binding to PLs containing SecYEG-Atto647N (D) is visible as co-localizing signal. In presence of SecYEG-PLs non-specific binding of SecA-FM to the gel fibers is eliminated (A,C).
