Figure 6. Abundance of AmelTwdl1, AmelTwdl2 and Ampxd transcripts in the integument of ligated (L) abdomens compared to non-ligated controls (NL).

Transcript levels were investigated at days (d) 1, 2, 3, 4, 5 and 6 after the abdominal ligature of newly ecdysed pupae. Transcript levels were assessed by semiquantitative RT-PCR assays followed by electrophoresis of the amplified cDNAs in ethidium bromide stained agarose gels. The Amactin gene was used as endogenous control.