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. 2011 May 31;6(5):e20532. doi: 10.1371/journal.pone.0020532

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Cal27 (blue triangles), Hep2 (purple triangles), HN6 (green squares), HN12 (purple squares), and HN30 (yellow diamonds) cells were incubated with increasing concentrations of wildtype PA in combination with FP59 (A), intercomplementing PA (PA-U2-R200A + PA-L1-I210A) in the presence of FP59 (B), wildtype PA with LF (C) or intercomplementing PA with LF (D) for 48 h. The cell viability was then measured using an MTT assay. HT29 colon carcinoma (grey circles) and HeLa (red circles) cells were used as a positive and negative controls, respectively [52], [74]. Cell survival is expressed as mean viability ± standard deviation of the mean.