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. 2011 May 31;6(5):e20590. doi: 10.1371/journal.pone.0020590

Figure 3. Assessment of apoptosis by TUNEL assay, flow cytometry and cell signaling in bone marrow cells of guinea pigs.

Figure 3

(Panel A) TUNEL assay of bone marrow cells. The rows were stained with fluorescein labeled dUTP and 4, 6-diamidino-2-phenylindole (DAPI), respectively; green fluorescence indicates TUNEL positive cells (magnification 200×). (Panel B) Quantitative evaluation of TUNEL positive cells; bars (means ± SD, n = 6) over the respective columns represent TUNEL positive cells, * significantly different (p<0.05) with respect to 0 day, ** significantly different (p<0.05) from 14 days treatment. (Panel C) Flow cytometry analyses of bone marrow cells using Annexin V-FITC fluorescence (Y-axis) vs PI (X-axis); quadrants: lower left, viable cells; upper left, apoptotic cells; upper right, late apoptotic and lower right, necrotic cells. (Panel D) p53 status in the bone marrow cells at different time. (Panel E) and (Panel F) Immunoblots of p-p53 (phospho-p53), Bax, Bcl-2, caspase 3 (C3), cleaved caspase 3 (CC3). (Panel G) and (Panel H) Immunoblots of TNF-α, caspase 8 (C8) and cleaved caspase 8 (CC8). Vit C means vitamin C.