Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May 31;6(5):e20612. doi: 10.1371/journal.pone.0020612

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Fan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Drosophila wt (upper panel) or dGe-1^Δ5 GLC (lower panel) ovarian extracts treated with cycloheximide and analyzed by centrifugation in a 10–45% sucrose density gradient. osk and actin mRNAs in the gradient fractions were detected by RNase protection assay. Absorption at 260 nm was measured to situate the monoribosome peak (80S) and polysomes. (B) Quantification of the RNase protection assays presented in (A). The relative levels of osk and actin mRNAs present in each fraction are indicated as a % of the total levels of both mRNAs present in wt (upper panel) and dGe-1^Δ5 GLC (lower panel) ovarian extracts. (C–D) Distribution of Stau protein in starved wt (C) or dGe-1^Δ5 GLC (D) ovaries. In nutritionally restricted wt oocytes, Stau (green) is present in large particles, which fail to form in starved dGe-1^Δ5 GLC ovaries. Bar, 50 µm.