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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Anal Chem. 2011 May 3;83(11):4118–4125. doi: 10.1021/ac200267t

Figure 3.

Figure 3

Microarray device alignment and characterization. A) Photograph of a microarray device with arrayed chemical-laden hydrogels (red arrow) sandwiched on to arrayed microwells (yellow arrow). Alignment features are indicated with black arrows. B, C) Phase contrast images with overlaid fluorescent images of an aligned device (scale bar = 100 μm). D, E, F) Phase contrast and fluorescent images, and quantification of fluorescence of released chemicals (green is FITC-labeled dextran and red is Rhodamine B). G–K) MCF-7 viability in sealed microwells at 0, 6, 12 and 24 hour time points (Live/Dead staining with calcein-AM, green, and ethidium homodimer, red).