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. Author manuscript; available in PMC: 2012 Apr 1.

Published in final edited form as: Neurogastroenterol Motil. 2011 Feb 9;23(4):379–e164. doi: 10.1111/j.1365-2982.2011.01675.x

Effect of devitalized tissue injury on gastrointestinal transit. Panel 1-A: Increasing graded amounts of tissue injury produced by the subcutaneous dorsal trans-implantation of a tissue-bone matrix (TBX) into a host caused a progressive delay in gastrointestinal transit as measured by the gastrointestinal distribution of an orally fed bolus of a non-digestible, non-absorbable FITC-dextran (70 kD) after 75 minutes. Sham implantation with subcutaneous administration of antibiotics did not delay gastrointestinal transit. Increasing the amount of tissue injury based on % body weight caused a marked delay in transit in TBX_15% and TBX_17.5% groups of mice measure 21 hours after implantation. Panel 1B: Calculated geometric center values from gastrointestinal transit data demonstrates a progressive delay in gastrointestinal transit with increasing graded amounts of tissue injury (TBX) after 21 hours of exposure with a significant transit delay using TBX_15% and a severe stasis using TBX_17.5%. Trans-implantation of TBX in amounts greater than 17.5% was lethal to the mice (N=4). Experimental manipulation of the TBX before trans-implantation by heat inactivation or de-cellularization completely prevented the delay in transit caused by TBX_17.5% and the observed mortality with TBX_20%, respectively.

Effect of devitalized tissue injury on gastrointestinal transit. Panel 1-A: Increasing graded amounts of tissue injury produced by the subcutaneous dorsal trans-implantation of a tissue-bone matrix (TBX) into a host caused a progressive delay in gastrointestinal transit as measured by the gastrointestinal distribution of an orally fed bolus of a non-digestible, non-absorbable FITC-dextran (70 kD) after 75 minutes. Sham implantation with subcutaneous administration of antibiotics did not delay gastrointestinal transit. Increasing the amount of tissue injury based on % body weight caused a marked delay in transit in TBX_15% and TBX_17.5% groups of mice measure 21 hours after implantation. Panel 1B: Calculated geometric center values from gastrointestinal transit data demonstrates a progressive delay in gastrointestinal transit with increasing graded amounts of tissue injury (TBX) after 21 hours of exposure with a significant transit delay using TBX_15% and a severe stasis using TBX_17.5%. Trans-implantation of TBX in amounts greater than 17.5% was lethal to the mice (N=4). Experimental manipulation of the TBX before trans-implantation by heat inactivation or de-cellularization completely prevented the delay in transit caused by TBX_17.5% and the observed mortality with TBX_20%, respectively.