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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2011 Mar 10;125(3-5):202–210. doi: 10.1016/j.jsbmb.2011.03.001

Figure 4. Testing variants with Chemical Complementation.

Figure 4

Chemical complementation in adenine selective media (-ALW) with wild-type hVDR and hVDR mutants. The variants tested are H305F, H305Y, and H305FH397Y. Gal4 is a ligand-independent transcription factor used as a positive control. All variants were tested for growth in the presence of LCA (A), 1,25(OH)2D3 (B), and cholecalciferol (C) with ligand concentrations ranging from 10−9 M − 10−5 M. With lithocholic acid all three variants display growth at 1 µM, whereas with cholecalciferol growth is only observed with H305F/H397Y at 10 µM. All of the variants and wild-type hVDR show growth at 10 nM 1,25(OH)2D3. The 10−10 M data point actually contains no ligand.