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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2011 Mar 10;125(3-5):202–210. doi: 10.1016/j.jsbmb.2011.03.001

Table 1.

EC50s and fold activation (FA) of wthVDR and variants

Lithocholic Acid
HEK293T 		Cholecalciferol
HEK293T
Variant EC50 FA EC50 FA
Wild-Type 100 µM 13 + 7 >100 µM 3 + 1
H305F 10 µM 40 + 14 3 µM 54 + 11
H305Y 10 µM 74 + 25 3 µM 65 + 6
H305F/H397Y 10 µM 84 + 14 0.3 µM 70 + 11

EC50 values, which are based on activation, and fold activation represent the average of two experiments in triplicate using HEK293T cells. EC50 values were extrapolated two ways. Method one used the best fit line of the raw data. Method two used a non-linear regression from GraphPad Prism. The fold activation was calculated by taking the highest level of activation and dividing this by the basal (no ligand) activation level. The error was obtained by calculating the standard deviation: σ = Square root (Σ[(X-μ)2]).