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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2011 Mar 8;127(6):1595–1603.e9. doi: 10.1016/j.jaci.2011.01.049

Figure 3. Physiological differences between control and IL-4 induced fibroblast-like cells derived from hESCs.

Figure 3

Figure 3

Figure 3

Figure 3

(A) Collagen gel contraction. The size of gels was measured on day 3 and shown as percentage of initial area. (B) Chemotaxis towards fibronectin. Chemotaxis was assessed by counting the number of migrated cells in five high-power fields (HPF). (C) Migration of fibroblast-like cells in the wound closure assay. The cell migration is expressed as a percentage of the cell occupied area in the initial wound area. (D) Cell proliferation. Cells were plated at a density of 2 × 104 cells/well in and cultured. Cell numbers were determined on days 3 and 5 using an electronic cell counter. For all experiments, three different batches of control and IL-4 induced cells were evaluated on three separate occasions, each performed in triplicate. Values are mean ± SD. * p < 0.05, ** p < 0.01.