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. Author manuscript; available in PMC: 2012 Jun 30.
Published in final edited form as: Vet Parasitol. 2011 Feb 26;179(1-3):137–143. doi: 10.1016/j.vetpar.2011.01.062

Figure 1.

Figure 1

Effect of RNA synthesis inhibitors on Ancylostoma caninum L3 activation in vitro. Increasing concentrations of mRNA synthesis inhibitors were co-incubated with canine serum filtrate and S-methyl-glutathione (GSM) in triplicate samples. The inset represents curve fitting to dose response models using GraphPad PRISM (ver 4.01) for the calculation of IC50 values. A) Effect of α-amanitin on L3 feeding. The sigmoidal dose response model was used for IC50 calculation. B) Effect of actinomycin D on L3 feeding. The sigmoidal dose response model with variable slope was used for IC50 calculation. Each experiment was repeated at least once.