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. Author manuscript; available in PMC: 2012 Jun 30.
Published in final edited form as: Vet Parasitol. 2011 Feb 26;179(1-3):137–143. doi: 10.1016/j.vetpar.2011.01.062

Figure 2.

Figure 2

Effect of protein synthesis inhibitors on Ancylostoma caninum L3 activation in vitro. Increasing concentrations of protein synthesis inhibitors were co-incubated with canine serum filtrate and GSM in triplicate samples. The inset represents curve fitting to dose response models using GraphPad PRISM (ver 4.01) for the calculation of IC50 values. A) Effect of puromycin on L3 feeding. The sigmoidal dose response model with variable slope was used for IC50 calculation. B) Effect of cycloheximide on L3 feeding. The sigmoidal dose response model was used for IC50 calculation. Each experiment was repeated at least once.