Table 1. Cell-cycle parameters of Tis21-GFP – and Tis21-GFP + APs and BPs in E14.5 mouse neocortex.
|
Cell-cycle phases (h) |
Growth fraction (%±s.e.m.) | ||||||
|---|---|---|---|---|---|---|---|
| TC-TS | TS | TC | TG2 | TM | TG1 | ||
| APs (Pax6+/Tbr2−) | 14.1 | 5.0 | 19.1 | 1.6 | 0.9 | 11.6 | 98±1 |
| Tis21-GFP− | 14.1 | 8.3 | 22.4 | 1.6 | 1.1 | 11.4 | 98±1 |
| Tis21-GFP+ | 14.0 | 1.8 | 15.8 | 1.6 | 0.7 | 11.7 | 99±0.5 |
| BPs (Tbr2+/Tbr1−) | 23.3 | 3.2 | 26.5 | 1.6 | 0.5 | 21.2 | 99±1 |
| Tis21-GFP− | 23.0 | 6.4 | 29.4 | 1.6 | 0.5 | 20.9 | 99±1 |
| Tis21-GFP+ | 23.4 | 2.8 | 26.2 | 1.6 | 0.5 | 21.3 | 99±1 |
| Tis21-GFP− NPCs | 8.0 | 23.3 | 1.6 | 1.0 | 12.7 | ||
|
Tis21-GFP+ NPCs |
|
2.4 |
21.7 |
1.6 |
0.6 |
17.1 |
|
AP and BP cell-cycle parameters were calculated from the data of Figures 2e, f and 4e and from the percentage of NPCs in M-phase. Average values for Tis21-GFP− and Tis21-GFP+ NPCs were calculated taking into account the relative proportions of NPC populations in VZ+SVZ (see Supplementary Fig. S2). TC, total cell cycle; TS, S-phase; TG2, G2-phase; TM, M-phase; TG1, G1-phase.