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. 2011 Feb 8;2:191. doi: 10.1038/ncomms1179

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(a) Schematic drawing of the positions of the UBA1 and UBA2 domains in wild-type Rad23 (Rad23^UBA1/UBA2), Rad23^UBA1/UBA1 and Rad23^UBA2/UBA1. (b) Steady-state levels of FLAG-tagged Rad23^UBA1/UBA2 (wild-type), Rad23^UBA1/UBA2* (Rad23^L392A), Rad23^UBA1/UBA1 and Rad23^UBA2/UBA1 were determined with a FLAG-specific antibody. Arrows indicate^FLAGRad23 bands. β-Actin is shown as loading control. Molecular weight marker is indicated. Asterisk indicates a non-specific band.