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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Mol Cell. 2011 May 6;42(3):342–355. doi: 10.1016/j.molcel.2011.04.011

Figure 1.

Figure 1

Identification of TFII-I as an OCA-B interacting protein. (A) Immobilized OCA-B interacts with transcription factor TFII-I in B cell nuclear extracts. Bacterially purified S-tagged OCA-B was immobilized on S-protein agarose and incubated with Namalwa B cell nuclear extracts. Bound proteins were analyzed by SDS-PAGE and silver staining. Protein bands indicated by arrows were analyzed by MS/MS. (B) Bound proteins in (A) were detected by immunoblot with a specific anti-TFII-I antibody. (C) Immunoblot analysis of anti-TFII-I and control rabbit IgG immunoprecipitates (IP) from Namalwa B cell nuclear extract with antibodies against proteins indicated on the right. Input represents 2.5% of total. (D) Immunoblot analysis of anti-FLAG immunoprecipitates from 293T cells transfected with vectors expressing OCA-B and/or FLAG-TFII-I. Input represents 5% of total.