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Zn2+, but not Mg2+, inhibits EtBr intercalation into duplex DNA containing consecutive FdU–dA base pairs. EtBr exclusion data are shown for each of the four DNA hairpins in the presence of the indicated concentrations of Zn2+ (red) or Mg2+ (blue). (A) 3′FdU hairpin; (B) 5′ FdU hairpin; (C) Alt-FdU hairpin; (D) No-FdU sequence.
