Table 1.

Binding constants for peptide-RNA binding of two different peptide mutants relative to the WT peptide

	R13A	K7A R13A
kobs (mutant): kobs (WT) [−]	0.43 ± 0.10	0.16 ± 0.04
KD (mutant): KD (WT) [−]	1.23 ± 0.13	2.27 ± 0.31
h (mutant): h (WT) [−]	1.07 ± 0.16	0.87 ± 0.10

Relative K_D values and Hill factors h were calculated from three independent sets of filter binding experiments. For comparison, the k_obs for mutant peptide accelerated annealing relative to the wild-type reaction constant is additionally listed.