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Thermodynamic data at 20°C derived from the Arrhenius plot in Figure 6
| K (M−1s−1) | Ea (kJ/mol) | ΔG≠ (kJ/mol) | ΔH≠ (kJ/mol) | ΔS≠ [J/(mol·K)] | |
|---|---|---|---|---|---|
| RNA only | 4.2 × 105 | 40 ± 1 | 40 ± 1 | 38 ± 1 | −8 ± 6 |
| Tat(44–61) 300 nM | 4.0 × 106 | 70 ± 6 | 35 ± 1 | 68 ± 6 | 112 ± 26 |
Parameters are as follows: k, RNA concentration corrected rate constant of the annealing reaction; Ea, Arrhenius activation energy; ΔG≠, free activation energy; ΔH≠, enthalpy; ΔS≠, entropy.
