Table 1.
DNA | Ionic conditions | Ncruciform (bp) | d(ln kr)/dn (kBT/turn) | d(ln kf)/dn (kBT/turn) | d(ln kr)/d (ln kf) = nr/nf | DNA unwinding in TS (bp) |
---|---|---|---|---|---|---|
WT Charomid X | 10 mM Phosphate buffer | 34 ± 1 (74) | 0.71 ± 0.014 | 0.31 ± 0.015 | 2.3 ± 0.1 | 10 ± 1 |
10 mM Phosphate Buffer + 50 mM NaCl | 36 ± 1 (58) | 0.30 ± 0.01 | 0.10 ± 0.01 | 2.9 ± 0.6 | 9 ± 1 | |
Mutated Charomid X | 10 mM Phosphate buffer | 34 ± 1 (51) | 0.91 ± 0.02 | 0.43 ± 0.02 | 2.1 ± 0.11 | 11 ± 1 |
10 mM Phosphate buffer + 50 mM NaCl | 36 ± 1 (65) | 0.11 ± 0.02 | 0.11 ± 0.02 | 1.0 ± 0.25 | 18 ± 3 | |
ColE1-5b | 11 mM Phosphate buffer | 30 ± 1 | 0.39 ± 0.05 | 1.68 ± 0.05 | 4.3 ± 0.5 | 5.8 ± 0.8 |
ColE1-8b | 11 mM Phosphate buffer | 32 ± 1 | 0.61 ± 0.13 | 1.57 ± 0.13 | 2.6 ± 0.5 | 9.1 ± 1.5 |
For unwinding amplitudes, the number in parenthesis is the number of events used to calculate the average.