Figure 2.

Defects in respiratory chain function upon TEFM gene silencing. (A) Western blot analyses of steady-state protein level of TEFM and subunits of respiratory chain complexes in control cells (untransfected cells and siRNA GFP) and cells treated with siRNA TEFM for 3 and 6 days. (B) Oxygen consumption rate (OCR) measured in quadruplicate population of control cells transfected siRNA GFP (green), cells treated with siRNA TEFM for 3 days (siRNA 1 in dark blue and siRNA 2 in light blue) and cells lacking mtDNA (Rho0, yellow). (C) Respiratory control ratio (RCR) in cells treated with siRNA GFP or siRNA TEFM for 3 or 6 days.