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. 2011 Jan 27;39(10):4284–4299. doi: 10.1093/nar/gkq1224

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Functional interaction of TEFM with RNA. (A) SDS–PAGE analysis of the affinity purified TEFM complex (lanes 1–3) treated with DNase I (lanes 4–6) lanes or RNase A (lanes 7–9) prior to the loading on the Strep-Tactin column. M, mitochondrial lysate; FT, flow through; E2, elution fraction 2; single asterisk, DNase I; two asterisks, RNase A. (B) Western blotting analysis of the SDS–PAGE gel shown in (C). The blot was incubated with the antibodies indicated to the right of the panel. (C) The co-localization of the HA-tagged version of TEFM with mtDNA and mtRNA analysed by immunofluorescence in A549 cells as described in ‘Materials and Methods’ section. Incorporation of BrU in RNA was visualized in cultured cells with BrU-specific mAbs. Black and white images shown in the top row were pseudo-coloured in red or green as indicated in the top right corner of each image and digitally overlaid; yellow staining is indicative of co-localization.