Figure 2.

Synthesis of the YafP–YFP protein fusion. A translational fusion between yafP gene and the yellow fluorescent protein (YFP) was used to monitor the synthesis of the YafP–YFP protein fusion in cells. (A) Growth curve of untreated cells. a, b and c represent three different time points at which the cells were sampled for (B) measurement of fluorescence using FACS analysis. (C) Cells sampled at b time point were treated with 1 µg/ml of the SOS-inducing DNA damaging agent Mitomycine C (MMC) and fluorescence was measured 30 min and 45 min after. Experiments were repeated three times. Representative curves are shown.