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. 2011 Feb 1;39(10):4330–4339. doi: 10.1093/nar/gkr022

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — mCreI and DNA target site structure. (A) mCreI is a monomerized version of the native I-CreI homodimeric LAGLIDADG homing endonuclease protein shown here (30). (B) Sequence of the native I-CreI/mCreI target site with positions of phosphodiester backbone cleavages in the top (downward filled triangle) and bottom (upward filled triangle) strands indicated on the sequence of the top strand of native target site DNA. Target site cleavage across the minor groove leaves 4-base, 3′-OH extended cohesive ends. Note the partially palindromic nature of the native target site sequence. (C) mCreI makes both direct and water-mediated contacts with target site DNA predominantly via amino acid residues located predominantly in β-sheets that lie in the major groove of target site DNA. The contact map is an updated and redrawn version of the original contact map shown in (34).