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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Oral Microbiol. 2010 Nov 18;26(1):2–18. doi: 10.1111/j.2041-1014.2010.00581.x

Figure 4.

Figure 4

Hydrogen peroxide killing assays. Streptococcus mutans cultures were prepared similarly as those for acid-tolerance assays. Following washes with 0.1 M glycine buffer, pH 7.0, the bacterial cells were incubated in glycine buffer containing 0.2% (volume/volume, 58 mM, final concentration) hydrogen peroxide for 90 min. The survival rates were determined by serial dilutions and plating on brain–heart infusion agar plates. Data presented here are representatives of more than three independent experiments. Solid symbols represent planktonic cultures and open symbols represent biofilms (BF).